Raf激酶抑制蛋白、基质金属蛋白酶-9mRNA在前列腺癌中的表达变化及意义

任乐; 杨栋; 赵鹏程; 田沛; 何朝宏

doi:10.13201/j.issn.1001-1420.2019.07.008

Raf激酶抑制蛋白、基质金属蛋白酶-9mRNA在前列腺癌中的表达变化及意义

- 郑州大学附属肿瘤医院河南省肿瘤医院泌尿外科(郑州, 450008)
基金项目:
河南省科技厅普通科技攻关项目（编号162102310318）

详细信息

通讯作者: 何朝宏,E-mail:hchzdey@126.com

中图分类号: R737.25

收稿日期: 2018-10-08

Expression and significance of matrix metalloproteinase-9 and Raf kinase inhibitor protein mRNA in prostate cancer

- Department of Urology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, 450008, China

More Information

Corresponding author: HE Chaohong, E-mail: hchzdey@126.com

Received Date: 08 October 2018

摘要

摘要: 目的：观察Raf激酶抑制蛋白（RKIP）、基质金属蛋白酶-9（MMP-9） mRNA在前列腺癌（PCa）中的表达变化，并探讨其临床意义。方法：采用qPCR法检测PCa及良性前列腺增生（BPH）组织中RKIP、MMP-9mRNA的表达，并分析二者表达与临床病理特征关系及二者之间的相关性。结果：RKIP mRNA在PCa组织中相对表达量显著低于BPH组织（P<0.01）；MMP-9mRNA在PCa组织中相对表达量显著高于BPH组织（P<0.01）。RKIP mRNA在PCa组织中相对表达量随着Gleason评分的增高而降低（均P<0.05）；在PCa组织T₂N₀M₀期以内的无侵袭转移组中的相对表达量高于侵袭转移组（P<0.05）。MMP-9mRNA在PCa组织中相对表达量随着Gleason评分的增高而增高（P<0.05）；在PCa组织T₂N₀M₀期以内的无侵袭转移组中的相对表达量低于侵袭转移组（P<0.01）。RKIP mRNA与MMP-9mRNA的表达呈负相关（P<0.01）。结论：RKIP mRNA低表达与MMP-9mRNA高表达可能与PCa的发生发展有关，同时RKIP mRNA与MMP-9mRNA表达之间具有相关性，RKIP可能通过调控MMP-9从而促进PCa的发生发展。
- 前列腺癌 /
- Raf激酶抑制蛋白 /
- 基质金属蛋白酶-9
Abstract: Objective: To investigate the expression and significance of Raf kinase inhibitor protein (RKIP) and matrix metalloproteinase-9 (MMP-9) mRNA in prostate cancer.Method: Real-time fluorescence quantitative PCR (qPCR) was used to determine the expressions of RKIP and MMP-9mRNA in prostate cancer and benign prostatic hyperplasia tissues, and the relationship between the expression and patients'clinicopathologic characteristics and the correlation between the two were analyzed.Result: The relative expression level of RKIP mRNA in prostate cancer tissues was lower than that in prostate hyperplasia tissues (P<0.01).The relative expression level of MMP-9mRNA in prostate cancer tissues was higher than that in prostate hyperplasia tissues (P<0.01).The relative expression level of RKIP mRNA in prostate cancer tissues decreased with the increase of Gleason score (P<0.05).The relative expression level of the non-metastatic group within T₂N₀M₀was higher than that of the invasive and metastatic group (P<0.05).The relative expression level of MMP-9mRNA in prostate cancer tissues increased with the increase of Gleason score (P<0.05).The relative expression level of the non-metastatic group within T₂N₀M₀was lower than that of the invasive and metastatic group (P<0.01).RKIP mRNA was negatively correlated with MMP-9mRNA (P<0.01).Conclusion: Low expression of RKIP mRNA and high expression of MMP-9may be related to the tumorigensis and progression of prostate cancer, and the expression of RKIP mRNA and MMP-9mRNA are correlated.RKIP may promote the occurrence and development of prostate cancer by regulating MMP-9.
- prostate cancer /
- Raf kinase inhibitor protein /
- matrix metalloproteinase-9

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