Abstract: Objective: To study the safety and efficacy of transurethral resection of bladder tumor following submucosal dissection of tumors using endoscopic injection needle with cystoscopy. Method: Between January 2017 and December 2018, sixty-three patients with non-muscle-invasive bladder cancer diagnosed by imaging or cystoscopy were included in Urological Surgery Department of People's Hospital of Quzhou. Submucosal dissection of bladder tumors was performed utilizing injection needleswith cystoscopy, followed by transurethral resection of bladder tumor.The duration of operation, blood loss, the duration of bladder irrigation, catheter retention time, postoperative hospitalstay, pathological grade and tumor recurrence rate after 6 months and 12 months were recorded. Result: Among them,61 cases were successfully completed the operation. Another 2 cases underwent conventional TURBT due to bleeding and unclearfield of vision. Diameter of tumors ranged from 1.0 cm to 4.0 cm. Tumors were removed by en-bloc resectionin 58 cases and the remaining 5 cases received piecemeal resection.Operation time was(46.0±18.2)minutes, and bladder irrigation time was(23±8)hours. Urinary catheter retention time was(4.8±1.8)days, and hospital stay was(6.1±1.8) days.None of the cases required blood transfusion. No perforation, postoperative bleeding or other complications happened except that 1 case presented obturator nerve reflex. Postoperative pathology: 47 patients were in T_a stage, 14 in T₁ and 2 in T₂.None of the 48 cases had tumor recurrence over the 6 months of follow-up period.Among them, 29 cases were followed up for 12 months, and 2 of themhad been reported to relapse.Conclusion: It is a safe and effective way to treat the non-muscle-invasive bladder tumor withtransurethral resection of bladder cancer following the submucosal dissection of bladder tumor usingcystoscopic injection needle.The complete resection of tumor is beneficial to the evaluation of pathology with more precise guiding for subsequent treatment.Complete removal of the bladder tumor is more consistent with the principles of surgical management. Though our surgical methods need to be performed step by step, the equipmentis easily accessed and the operation is easy to be carried out, especially in the primary hospital, which is one of the advantages of this operation.