Inhibition of chloroquine on the proliferation of prostate cancer cell line PC3 in high glucose condition
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摘要: 目的：研究氯喹是否能通过自噬和活性氧（ROS）通路抑制高糖诱导的前列腺癌PC3细胞的增殖。方法：PC3细胞培养后分为4组（正常糖组、高糖组、正常糖+氯喹组、高糖+氯喹组）予相应干预。运用CCK-8实验检测细胞的增殖能力；Western blot检测自噬相关蛋白LC3的表达；DCFH-DA活性氧荧光探针检测细胞中ROS水平；JC-1法检测细胞线粒体膜电位的去极化。结果：CCK-8实验结果表明氯喹能够显著抑制高糖环境下PC3细胞的增殖。Western blot实验结果显示氯喹会使PC3细胞LC3-Ⅱ/Ⅰ比值升高，说明细胞自噬显著被抑制。DCFH-DA探针检测结果表明，氯喹能够显著上调高糖环境下PC3细胞中ROS水平。JC-1检测结果表明，氯喹能够诱导高糖环境下PC3细胞中线粒体膜电位去极化。结论：氯喹通过自噬和ROS通路抑制高糖环境下前列腺癌细胞的增殖。Abstract: Objective: To investigate whether chloroquine can inhibit the proliferation of prostate cancer cell line PC3 in high glucose condition via autophagy and reactive oxygen species(ROS) pathways.Methods: PC3 cells were divided into four groups:normal glucose group, high glucose group, normal glucose+chloroquine group and high glucose+chloroquine group. CCK-8 assay was applied to assess the effect of proliferation. The protein expression of LC3 related to autophagy was detected with Western blot. ROS levels were detected using DCFH-DA staining in PC3 cells. JC-1 staining was performed to investigate the depolarization of mitochondrial membrane in PC3 cells.Results: CCK-8 assay showed that the proliferation of PC3 cells were significantly inhibited by chloroquine in high glucose condition. Western blot assay indicated that the LC3-Ⅱ/Ⅰexpression of PC3 cells were elevated by chloroquine in high glucose condition, which illustrated that chloroquine inhibited the autophagy of PC3 cells. DCFH-DA staining assay showed that the ROS levels in PC3 cells were up-regulated by chloroquine in high glucose condition. Furthermore, results of JC-1 assay demonstrated that chloroquine induced the depolarization of mitochondrial membrane of PC3 cells in high glucose condition.Conclusion: Chloroquine can inhibit the proliferation of prostate cancer cell line PC3 in high glucose condition via autophagy and ROS pathways.
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Key words:
- prostate carcinoma /
- chloroquine /
- autophagy /
- reactive oxygen species /
- hyperglycemia
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