Expression and Clinical Significance of Hyaluronan Synthase 2(HAS2) in Renal Carcinoma
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摘要: 目的：研究透明质酸合成酶2在肾癌中的表达，并探讨其潜在的临床意义。方法：运用实时定量聚合酶链反应（qPCR）方法检测五种肾癌细胞（ACHN、Caki-1、OS-RC-2、786-O、SN12PM6）透明质酸合成酶三种亚型（HAS1、HAS2、HAS3） mRNA的表达，运用Western blot方法进一步检测mRNA表达含量高的HAS亚型在五种肾癌细胞系及肾透明细胞癌（ccRCC）组织中的蛋白表达。结果：在五种肾癌细胞系中HAS2mRNA表达水平均明显高于正常肾小管上皮细胞（HK-2），其中在肾癌SN12PM6细胞系中表达水平最高（均P<0.05），HAS1mRNA的表达水平均明显低于正常肾小管上皮细胞（均P>0.05），而HAS3mRNA表达水平除肾癌Caki-1细胞系外均低于正常肾小管上皮细胞（P>0.05）。在五种肾癌细胞系中HAS2蛋白均明显表达，而正常肾小管上皮细胞无明显表达。在肾透明细胞癌组织中HAS2蛋白表达也明显高于相应癌旁组织。结论：透明质酸合成酶2在多种肾癌细胞系和肾透明细胞癌组织中均明显表达，提示透明质酸合成酶2可能在肾癌尤其在肾透明细胞癌发生发展过程中起着至关重要的某种作用，并可能成为新的肾透明细胞癌分子标志物。Abstract: Objective: To study the expression of HAS2 in renal carcinoma and discuss the potential clinical significance. Method: qPCR was applied to detect the expression of HAS1 mRNA, HAS2 mRNA and HAS3 mRNA in five ccRCC cell lines (ACHN, Caki-1, OS-RC-2, 786-O, SN12PM6) and human renal proximal tubular cell line(HK-2 cells). Additionally, Western blot was taken to examine the expression of the increased HAS protein in above renal cancer cells and ccRCC tissues.Result: HAS2 mRNA expression was obviously higher in the five ccRCC cell lines than that in the HK-2 cells, especially in the SN12PM6 cells(P<0.05). HAS1 mRNA expression reduced distinctly in the five ccRCC cell lines compared with the HK-2 cells(P>0.05). The expression of HAS3 mRNA was also lower in the renal cancer cell lines than that in the HK-2 cells except Caki-1 cells (P>0.05). The expression of HAS2 protein significantly increased in the five ccRCC cell lines.In addition, the expression of HAS2 protein was significant higher in the ccRCC tissue than those in the normal tissue(P<0.05). Conclusion: Hyaluronan synthase 2 was detected to highly express in the five ccRCC cell lines and ccRCC tissue,which shows that hyaluronan synthase 2 may play a crucial role in the develepment of renal carcinoma especially in ccRCC and may serve as a potential molecular marker for clear renal cell carcinoma.
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Key words:
- renal carcinoma /
- hyaluronan acid /
- hyaluronan synthase 2
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