N-乙酰半胱氨酸抑制大鼠肾草酸钙结石形成机制的研究

张雍偲; 刘春

N-乙酰半胱氨酸抑制大鼠肾草酸钙结石形成机制的研究

张雍偲,
刘春^,

- 山西医科大学第一医院泌尿外科(太原, 030001)

详细信息

通讯作者: 刘春,E-mail:sxtyliuchun@126.com

中图分类号: R692.4

收稿日期: 2012-12-02

Research the mechanism of N-acetyl cysteine inhibiting renal calcium oxalate stone formation in rats

ZHANG Yongcai,
LIU Chun^,

- Department of Urology, the First Hospital of Shanxi Medical University, Taiyuan, 030001, China

More Information

Corresponding author: LIU Chun, E-mail: sxtyliuchun@126.com

Received Date: 02 December 2012

摘要

摘要: 目的：探讨N-乙酰半胱氨酸（NAC）对大鼠肾草酸钙结石形成的影响及机制，为临床预防尿路结石提供理论依据。方法：将30只健康清洁成年雄性Wistar大鼠先在相同环境下适应性喂养1周，然后随机分为3组：A组（空白对照组）、B组（单纯诱石组）、C组（诱石+NAC干预组）。A组饮去离子水，B组饮1%乙二醇的去离子水，C组饮1%乙二醇去离子水，并给予NAC 100 mg/（kg·d）灌胃。第4周处死大鼠，取出双肾，左肾纵向剖开，用10%甲醛固定，HE染色石蜡切片，在100、400倍光镜下观察肾组织草酸钙结晶沉积情况，并进行分级及评分。右肾皮质制成10%的匀浆，检测丙二醛（MDA）及超氧化物歧化酶（SOD）。全部实验数据通过SPSS17.0统计软件分析处理。结果：①肾脏结晶沉积情况分级及评分结果：与B组相比，C组的肾脏结晶沉积评分明显降低（P<0.05）。②MDA检测结果：与B组相比，C组的MDA含量降低，差异有统计学意义（P<0.05）。③SOD检测结果：与B组相比，C组的SOD含量增高，差异有统计学意义（P<0.05）。④A、B、C三组肾组织结晶等级评分与SOD含量的相关系数为-0.499（P<0.01），结晶评分与MDA含量的相关系数为0.592（P<0.01）。结论：NAC可以通过其抗氧化作用抑制大鼠肾草酸钙结石的形成。
- 肾结石 /
- N-乙酰半胱氨酸 /
- 超氧化物歧化酶 /
- 丙二醛
Abstract: Objective: To study the effect and mechanism of N-acetyl cysteine(NAC) on renal calcium oxalate stone formation in rats, For clinical prevention urinary stones provide theoretical basis.Methods: At first, 30 health clean adult male Wistar rats adaptability feeding 1 week in the same environment,then randomly divided into 3 groups(n=10):group A(Blank control group), group B(stone forming group), group C(stone forming+NAC intervention group), group A drink deionized water, group B drink 1% glycol in deionized water, group C drink 1% glycol in deionized water, and to give NAC100 mg/(kg·d) by lavage. After feeding for 4 weeks,remove double kidney, left renal opened longitudinally,with 10% formaldehyde fixed, HE dyeing paraffin section, the deposit condition of calcium oxalate crystals in nephridial tissue was observed and scored by 100, 400 times light-microscopy. Right renal cortex made 10% of slurry, detection of MDA and SOD. All the experimental data analysis by SPSS17.0 statistical software.Results: ①Classification and score the deposit condition of calcium oxalate crystals in nephridial tissue:Compared with group B, group C of kidney crystal deposition score significantly decrease(P<0.05); ②MDA test results:Compared with group B, group C of MDA content decreased, difference have statistical significance(P<0.05); ③SOD test results:Compared with group B, group C of SOD content increased, difference have statistical significance(P<0.05); ④The correlation coefficient of score(deposit condition of calcium oxalate crystals in nephridial tissue) and SOD content is -0.499(P<0.01), the correlation coefficient of score and MDA content is 0.592(P<0.01).Conclusions: NAC can inhibiting renal calcium oxalate stone formation in rat through its antioxidation.
- renal calcium /
- NAC /
- superoxide dismutase /
- malondialdehyde

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