精浆弹性蛋白酶水平与精液主要参数和精子功能的相关性分析

刘雅峰; 欧建平; 钟依平; 徐艳文; 苗本郁; 李洁; 周灿权

精浆弹性蛋白酶水平与精液主要参数和精子功能的相关性分析

- 1 中山大学附属第一医院生殖医学中心(广州, 510080)
基金项目:
广东省科技计划项目(No:2010B031600081)

详细信息

通讯作者: 刘雅峰,E-mail:liuyang20030901@yahoo.com.cn

中图分类号: Q592.9;R698

收稿日期: 2011-02-05

Correlation of seminal plasma elastase and semen major parameters and sperm function

- Center for Reproductive Medcine, The First Affiliated Hospital of Sun Yat-Sen Unversituy, Guangzhou, 510080, China

More Information

Corresponding author: LIU Yafeng, E-mail: liuyang20030901@yahoo.com.cn

Received Date: 05 February 2011

摘要

摘要: 目的:探讨精浆弹性蛋白酶与精液主要参数和指标的关系。方法:用酶联免疫吸附法(ELISA)检测精浆中的弹性蛋白酶,按照WHO人类精液实验室手册要求进行精液常规分析、精子形态分析,检测精子顶体酶活性、精浆抗体(AsAb)、解脲支原体等,分析弹性蛋白酶与男性不育相关因素的关系。结果:209例男性不育患者中,43例患者精浆弹性蛋白酶 ≥ 290 ng/ml,设为炎症组;166例患者精浆弹性蛋白酶<290 ng/ml,设为非炎症组。炎症组的精子密度、精子活动率、a+b级活力精子率、精子顶体酶阳性率均低于非炎症组(P<0.05);而精子畸形率、精浆抗体(AsAb)、解脲支原体阳性率均高于非炎症组(P<0.05)。两组的精液量、PH值和液化时间差异无统计学意义。结论:精浆弹性蛋白酶水平与精液质量有密切的关系,生殖道感染是导致男性不育的重要原因。
- 精浆弹性蛋白酶 /
- 精液参数 /
- 男性不育
Abstract: Objective:To study the relationship between seminal plasma elastase and semen major parameters.Methods:The level of seminal plasma elastase was detected by ELISA(Enzyme-linked immunosorbent assay). Semen major parameters, abnormal morphological sperm rate, sperm acrosin reaction, AsAb and UU infection were measured and analyzed in 209 infertile men according to WHO laboratory handbook about human semen.Results:43 cases were men whose level of seminal plasma elastase was equal to or higher than 290 ng/ml in 209 male infertility, set up into inflammation group. The others (<290 ng/ml) were 166 cases, set up into non-inflammation group. Semen concentration, motility, a+b grade motility, and sperm acrosin reaction rate in inflammation group were lower than in non-inflammation group(P<0.05). Higher abnormal morphological sperm, UU infection rate and AsAb positive rate were observed in inflammation group(P<0.05). Sperm volume, PH value and liquefying time were not significantly different between inflammation and non-inflammation group(P>0.05).Conclusions:The level of seminal plasma elastase is associated with sperm quality, which can be considered as one of the important reasons resulting in male infertility.
- seminal plasma elastase /
- semen parameter /
- male infertility

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参考文献(6)

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