Notch1、Notch2及c-Myc在肾透明细胞癌中的表达及意义

黄庆波; 刘尚文; 艾青; 司马晋; 姚志勇; 张帆; 马鑫; 张旭

Notch1、Notch2及c-Myc在肾透明细胞癌中的表达及意义

- 中国人民解放军总医院军医进修学院泌尿外科(北京, 100853)
基金项目:
国家自然科学基金资助项目(No:30972982)

详细信息

通讯作者: 张旭,E-mail:xzhang@foxmail.com

中图分类号: R737.11

收稿日期: 2011-12-21

Expression and significance of Notch1、Notch2 and c-Myc in clear cell renal cell carcinoma

- Department of Urology, Chinese PLA General Hospital/PLA Military Postgraduate Medical School, Beijing, 100853, China

More Information

Corresponding author: ZHANG Xu, E-mail: xzhang@foxmail.com

Received Date: 21 December 2011

摘要

摘要: 目的:探讨Notch信号受体Notch1、Notch2及c-Myc在肾透明细胞癌中的表达与意义。方法:通过实时定量PCR及western-blot方法检测27例肾透明细胞癌及瘤旁组织中Notch1、Notch2及c-Myc的表达水平,并采用相关性分析检验Notch1、Notch2的表达与c-Myc的表达相关性。结果:Notch1、Notch2及c-Myc在肾癌组织中表达水平明显高于相应的癌旁组织(P<0.001),相关性分析表明在肿瘤组织中c-Myc与Notch2的mRNA水平呈正相关(r=0.629,P＜0.001),而与Notch1的mRNA水平无相关(P=0.389)。结论:Notch信号通路及c-Myc的异常高表达可能与肾透明细胞癌的发生发展有关,而且c-Myc可能作为Notch信号通路的下游靶基因,发挥促癌的作用。
- 肾肿瘤 /
- Notch受体 /
- c-Myc /
- 相关性分析
Abstract: Objective:To investigate the expression and significance of Notch1, Notch2 and c-Myc in clear cell renal cell carcinoma (ccRCC). Method:Message RNA and protein levels of Notch1, Notch2 and c-Myc were detected in 27 ccRCC tissue samples and their corresponding adjacent normal tissue samples by real-time PCR and western-blot, respectively. Correlation analyses were utilized between mRNA expressions of Notch1, Notch2 and c-Myc. Result:Notch1, Notch2 and c-Myc were all upregulated in ccRCC samples relative to adjacent normal tissue samples (P<0.001). Correlation analyses showed that c-Myc mRNA expression was closes correlated with Notch2 mRNA expression in ccRCC (r=0.629, P<0.001), but not with Notch1 mRNA expression (P=0.389).Conclusion:Up-regulated Notch1, Notch2 and c-Myc may associate with ccRCC and c-Myc may serve as a target gene of Notch pathway signaling.
- kidney neoplasms /
- Notch receptor /
- c-Myc /
- correlation analysis

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参考文献(8)

[1]	SJLUND J,JOHANSSON M,MANNA S,et al.Suppression of renal cell carcinoma growth by inhibi-tion of Notch signaling in vitro and in vivo[J].J ClinInvest,2008,118:217-228.
[2]	MORRISH F,NERETTI N,SEDIVY J M,et al.The oncogene c-Myc coordinates regulation of meta-bolic networks to enable rapid cell cycle entry[J].Cell Cycle,2008,7:1054-1066.
[3]	WENG A P,MILLHOLLAND J M,YASHIRO-OHTANI Y,et al.c-Myc is an important direct tar-get of Notch1in T-cell acute lymphoblastic leukemia/lymphoma[J].Genes Dev,2006,20:2096-2109.
[4]	SHARMA V M,DRAHEIM K M,KELLIHER MA.The Notch1/c-Myc pathway in T cell leukemia[J].Cell Cycle,2007,6:927-930.
[5]	TIEN A C,RAJAN A,BELLEN H J.A Notch up-dated[J].J Cell Biol,2009,184:621-629.
[6]	李林,罗进勇.Notch信号通路及其与肿瘤的关系[J].临床和实验医学杂志,2010,9(19):1509-1511.
[7]	程伟,金明,邵国兴,等.C-myc基因在肾癌组织中的表达[J].临床泌尿外科杂志,2001,16(4):178-180.
[8]	VITA M,HENRIKSSON M.The Myc oncoproteinas a therapeutic target for human cancer[J].SeminCancer Biol,2006,16:318-330.