B7-H1及其受体PD-1在膀胱癌患者外周血免疫细胞中的表达及其临床意义

纪传彪; 王永华; 于芹超; 刘静; 崔杰; 公欣海

B7-H1及其受体PD-1在膀胱癌患者外周血免疫细胞中的表达及其临床意义

- 1. 青岛大学医学院附属医院泌尿外科(山东青岛, 266000);
- 2. 青岛大学医学院附属医院儿科
基金项目:
国家自然科学基金青年科学基金项目（编号81101932）

详细信息

通讯作者: 王永华,E-mail:doctorwangyonghua@yahoo.cn

中图分类号: R737.14

收稿日期: 2012-11-24

Expressions and significance of B7-H1 and PD-1 in peripheral blood immune cells from patients with bladder cancer

- 1. Department of Urology, the Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, 266000, China;
- 2. Department of Pediatrics, the Affiliated Hospital of Medical College, Qingdao University

More Information

Corresponding author: WANG Yonghua, E-mail: doctorwangyonghua@yahoo.cn

Received Date: 24 November 2012

摘要

摘要: 目的：研究膀胱癌患者外周血中树突状细胞（dendritic cells， DCs）表面B7-H1和CD₈⁺T淋巴细胞表面PD-1的表达情况及其临床意义。方法：分离30例膀胱癌患者外周血单个核细胞（peripheral blood mononuclear cell， PBMC），联合培养后采用流式细胞术检测DCs表面B7-H1及CD₈⁺T细胞表面PD-1的表达情况。结果：膀胱癌患者外周血DCs表面B7-H1表达水平及CD₈⁺T细胞表面PD-1表达水平均明显高于正常人，差异具有显著统计学意义（P<0.01），并且二者的表达水平随着病理分级和临床分期的增加均升高，差异有统计学意义（P<0.05）。结论：在膀胱癌发生发展过程中存在着DCs表面B7-H1和CD₈⁺T细胞表面PD-1分子表达的上调，B7-H1/PD-1信号通路可能在T细胞免疫应答的初始阶段亦参与了膀胱癌免疫逃逸的发生。
- 膀胱肿瘤 /
- B7-H1 /
- PD-1 /
- 树突状细胞 /
- CD₈⁺T淋巴细胞 /
- 免疫逃逸
Abstract: Objective: To study the expressions and the clinical significance of B7-H1 on dendritic cell and PD-1 on CD₈⁺T lymphocytes cells in peripheral blood from patients with bladder cancer.Methods: Peripheral blood mononuclear cell were disparted from 30 bladder cancer patients and 10 healthy controls by density gradient centrifugation and then co-cultured. The expressions of B7-H1 on dendritic cells (DCs) and PD-1 on CD₈⁺T lymphocytes were analyzed by flow cytometry.Results: Expressions of B7-H1 on dendritic cells and PD-1 on CD₈⁺T lymphocytes in bladder cancer were higher than healthy controls (P<0.01). And the expressions were strongly associated with the pathological grade and clinical stage of bladder cancer (P<0.05).Conclusions: The upregulation of B7-H1 on DCs and PD-1 on CD₈⁺T lymphocytes were strongly associated with neoplastic progression of bladder cancer. B7-H1/PD-1 signal passway may also play an important role in immune escape of bladder cancer during initial phase of T cell immune response.
- bladder cancer /
- B7-H1 /
- PD-1 /
- dendritic cells /
- CD₈⁺t lymphocytes /
- immune escape

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