聚己内酯/丝素蛋白/胶原电纺纤维的制备及其细胞相容性研究

魏盖杰; 李超; 张心如; 李鸿宾

聚己内酯/丝素蛋白/胶原电纺纤维的制备及其细胞相容性研究

- 上海交通大学附属第六人民医院东院泌尿外科(上海, 200233)
基金项目:
国家自然科学基金（编号30901503；81370793）

详细信息

通讯作者: 李超,E-mail:chaoli1979@126.com

中图分类号: Q813

收稿日期: 2013-06-03

Preparation of polycaprolactone-silk fibroin-collagen electrospun ultrafine fibers and its biocompatibility

- Department of Urology, the Sixth People's Hospital, Shanghai Jiaotong University, Shanghai, 200233, China

More Information

Corresponding author: LI Chao, E-mail: chaoli1979@126.com

Received Date: 03 June 2013

摘要

摘要: 目的：制备聚己内酯/丝素蛋白/胶原电纺纳米纤维支架，检测其对口腔黏膜上皮细胞生长和增殖的影响。方法：将再生丝素膜、水溶性胶原蛋白粉末及聚己内酯按质量比1：1：4；1：1：8；1：1：10 共同溶于六氟异丙醇中，采用静电纺丝法制备制备聚己内酯/胶原/丝素蛋白电纺纳米纤维支架。将体外培养的口腔黏膜上皮细胞接种至材料表面，采用MTT法和扫描电镜研究口腔黏膜上皮细胞在材料表面的生长和增殖情况，评价聚己内酯/丝素蛋白/胶原电纺纳米纤维的细胞相容性。结果：MTT结果表明，口腔黏膜上皮细胞在聚己内酯/丝素蛋白/胶原电纺纳米纤维支架生长良好。电镜观察显示所制备的电纺纤维直径均一，呈相互连通的多孔网状结构，口腔黏膜上皮细胞在改性后的材料表面具有良好的生长形态。结论：聚己内酯/丝素蛋白/胶原电纺纳米纤维支架，具备合适的孔径和孔隙率，适合口腔黏膜上皮细胞生长，细胞相容性良好，是一种组织工程尿道重建良好的支架载体。
- 聚己内酯 /
- 丝素蛋白 /
- 胶原 /
- 纳米纤维 /
- 细胞相容性
Abstract: Objective: To prepare the polycaprolactone-silk fibroin-collagen electrospun nanofibrous scaffold and evaluate the growing and proliferation of oral keratinocytes on it.Method: The regenerated silk fibroin membrane, water-soluble collagen powder and polycaprolactone were dissolved in hexafluoro-isopropanol at the mass ratio of 1:1:4; 1:1:8; 1:1:10. Method of electrostatic spinning was used to prepare porous nanofiber scaffold of polycaprolactone-silk fibroin-collagen. The scaffold was observed by scanning electron microscope. The parameters of scaffold fiber diameter, porosity, water absorption rate, and tensile strength were detected. Oral keratinocytes were seeded on the electrospun scaffold and the cell proliferation was also evaluated by MTT method and scanning electron microscope.Result: The MTT results of biocompatibility showed that grafted collagen was obviously suitable for the growth of oral keratinocytes. The scanning electron microscope images showed that the fibers were smooth and uniform with the interwoven and porous morphology, and oral keratinocytes grew well on the scaffold.Conclusion: The nanofiber scaffold of polycaprolactone-silk fibroin-collagen prepared by the method of electrostatic spinning is safe, free of toxicity, and suitable for oral keratinocytes growth. It has good cytocompatibility and proper aperture and porosity, which is a potential scaffold carrier for tissue engineered urethra.
- polycaprolactone /
- silk fibroin /
- collagen /
- nanofiber /
- biocompatibility

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