丙戊酸钠增强免疫细胞对人膀胱癌细胞的杀伤作用

邓汪东; 杨峰涛; 丁国栋

doi:10.13201/j.issn.1001-1420.2014.06.029

丙戊酸钠增强免疫细胞对人膀胱癌细胞的杀伤作用

- 1. 中山大学附属梅州医院泌尿外四科(广东梅州, 514031);
- 2. 汕头大学医学院第一附属医院泌尿外科
基金项目:
广东省科技计划项目资助 (编号2010B031600279)

广东省医学科研基金资助项目 (编号A2010398)

详细信息

通讯作者: 杨峰涛,E-mail:dengziyang2003@163.com

中图分类号: R737.14

收稿日期: 2014-04-13

Sodium valproate enhances the cytotoxicity of immune cells to human bladder cancer cells

- 1. Department of Urology, Affiliated Meizhou Hospital of SUN Yat-sen University, Meizhou, Guangdong, 514031, China;
- 2. Department of Urology, First Affiliated Hospital, Shantou University Medical College

More Information

Corresponding author: YANG Fengtao, E-mail: dengziyang2003@163.com

Received Date: 13 April 2014

摘要

摘要: 目的:探讨丙戊酸钠(VPA)对膀胱癌细胞MICA表达的影响以及所产生的肿瘤免疫作用,以期为防治膀胱癌复发和浸润提供新的治疗方法。方法:采用不同浓度VPA处理人膀胱尿路上皮癌T₂₄细胞后,用半定量RT-PCR和流式细胞术检测癌细胞中MICA mRNA和蛋白表达。用乳酸脱氢酶法检测外周血单个核细胞(PBMCs)对经VPA处理的T₂₄细胞的杀伤作用。结果:VPA从mRNA和蛋白水平诱导T₂₄细胞表达MICA,并增强T₂₄细胞对PBMCs细胞毒作用的敏感性。结论:VPA能增强膀胱癌对免疫细胞杀伤作用的敏感性,可作为防治膀胱癌的辅助药物。
- 膀胱癌 /
- 丙戊酸钠 /
- NKG2D /
- MICA
Abstract: Objective:To investigate the effect of sodium valproate (VPA) on the expression of MICA and PBMCs-mediated cytotoxicity in T₂₄ bladder cancer cells so as to provide a new method for treatment and prevention of recurrent and infiltrating bladder cancer.Method:T₂₄ cancer cells were treated with VPA and the expression of MICA at the mRNA and protein levels were analyzed by RT-PCR and flow cytometry. Cytotoxicity of PBMCs against the T₂₄ cells was investigated with the lactate dehydrogenase release assay. Result:VPA increased MICA expression in T₂₄ cells and sensitizes cancer cells to cytotoxicity of PBMCs.Conclusion:VPA sensitizes human bladder cancer cells to cytotoxicity of immune cells.
- bladder cancer /
- sodium valproate /
- NKG2D /
- MICA

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