慢性非细菌性前列腺炎小鼠前列腺中α<sub>1</sub>-受体亚型的表达变化

刘峰; 许小林; 俞建军; 胡晓勇

doi:10.13201/j.issn.1001-1420.2017.02.012

慢性非细菌性前列腺炎小鼠前列腺中α₁-受体亚型的表达变化

- 1上海交通大学附属第六人民医院南院泌尿外科(上海, 201499);
- 2 上海交通大学附属第六人民医院泌尿外科
基金项目:
上海市卫计委青年基金项目（编号2011-Y132）

详细信息

通讯作者: 俞建军,E-mail:13916672582@163.com

中图分类号: R697.33

收稿日期: 2016-09-16

Expression of α₁-adrenoceptor subtypes in the mouse with chronic abacterial prostatitis

- 1 Department of Urology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201499, China;
- 2 Department of Urology, ShanghaiJiao Tong University Affiliated Sixth People's Hospital

More Information

Corresponding author: YU Jianjun, E-mail: 13916672582@163.com

Received Date: 16 September 2016

摘要

摘要: 目的：复制小鼠慢性非细菌性前列腺炎（CAP）模型，研究前列腺炎发展过程中α₁肾上素能受体（α₁-AR）亚型的表达及其与前列腺中炎症介质TNF-α的关系。方法：使用大鼠前列腺蛋白提纯液辅以双重免疫佐剂，造成实验性慢性非细菌性前列腺炎（CAP）小鼠模型，在造模后45天解剖小鼠，获得前列腺组织样本，抽提RNA转录为cDNA，运用实时荧光逆转录聚合酶链反应检测组织标本中α₁a-AR、α₁b-AR、α₁d-AR、TNF-α的mRNA的表达，观察CAP小鼠α₁-AR三种亚型的表达变化，并观察其与前列腺中炎症介质TNF-α的关系。背侧组织做病理切片观察，进一步判别前列腺的炎症程度。结果：造模小鼠均出现排尿障碍等症状；病理切片观察到炎症细胞浸润；造模小鼠中TNF-α明显升高，但α₁a-AR、α₁b-AR、α₁d-AR无明显变化。结论：慢性前列腺炎小鼠模型复制成功，CAP的炎症程度与TNF-α的表达成正相关关系，与α₁a-AR、α₁b-AR、α₁d-AR无明显关系。
- 慢性非细菌性前列腺炎 /
- 动物模型 /
- 免疫佐剂法
Abstract: Objective: To make a kind of chronic abacterial prostatitis(CAP) mice, and try to find the relationship between α₁-adrenoceptor(α₁-AR) subtypes and prostate inflammatory mediators TNF-α.Method: We use prostate protein purification solution from SD rat to cause a kind of experimental CAP mouse model with two immune adjuvants.We killed mice after 45 days to get the prostatits tissue samples.And then, RNA was extracted and transcribed into cDNA.Later, we used real time reaction to detect the expression of α₁a-AR, α₁bAR, α₁d-AR, TNF-α, and found the expression changes in three α₁-AR subtypes as well as the relationship with TNF-α.We also did some pathological observation to define the degree of inflammation in prostate.Result: The symptoms such as building urination disorders occurring in CAP mice and pathological inflammatory cell infiltration were observed.In realtime result, TNF-αhad a higher expression in CAP group, while α₁a-AR, α₁b-AR and α₁d-AR didnt show the significance.Conclusion: We have copied a kind of CAP mice.The degree of inflammation is positively related to the expression of TNF-α, but not associated with α₁a-AR, α₁b-AR or α₁d-AR.
- chronic abacterial prostatitis /
- animal models /
- immune adjuvants

HTML全文

参考文献(8)

[1]	Wittnam C A.The measure of the man[J].Ann Intern Med, 2008, 149(6):431-432.
[2]	Keetch D W, Humphrey P, Ratliff T L.Development of a mouse model for nonbacterial prostatitis[J].J Urol, 1994, 152(1):247-250.
[3]	叶伟成, 薛慈民, 徐兆东, 等.免疫佐剂法制作慢性非细菌性前列腺炎小鼠模型的方法[J].中国男科学杂志, 2001, 15(1):29-32.
[4]	Wang X, Zhong S, Xu T, et al.Histopathological classification criteria of rat model of chronic prostatitis/chronic pelvic pain syndrome[J].Int Urol Nephrol, 2015, 47(2):307-316.
[5]	Kojima Y, Sasaki S, Shinoura H, et al.Quantification of alpha1-adrenoceptor subtypes by real-time RT-PCR and correlation with age and prostate volume in benign prostatic hyperplasia patients[J].Prostate, 2006, 66(7):761-767.
[6]	Nickel J C.Antibiotics and alpha-blockers for chronic prostatitis:evidence from recent randomized placebocontrolled studies[J].Rev Urol, 2005, 7(1):65-66.
[7]	李宏军, 李汉忠, 商学军, 等.α受体阻滞剂治疗III型前列腺炎的效果分析[J].中华泌尿外科杂志, 2006, 27(6):424-427.
[8]	Alexander R B, Propert K J, Schaeffer A J, et al.Ciprofloxacin or tamsulosin in men with chronic prostatitis/chronic pelvic pain syndrome:a randomized, doubleblind trial[J].Ann Intern Med, 2004, 141(8):581-589.