OIP5基因在肾癌中的表达及作用

宫满成; 董文静; 丘少鹏; 袁润强

doi:10.13201/j.issn.1001-1420.2017.06.015

OIP5基因在肾癌中的表达及作用

- 1. 中山市人民医院泌尿外科(广东中山, 528403);
- 2. 中山市人民医院肿瘤内科;
- 3. 中山大学附属第一医院泌尿外科
基金项目:
广东省医学科研基金项目(编号A2016058)

中山市科技计划项目(编号2015B1012)

中山市医学科研基金项目(编号2016J040)

详细信息

通讯作者: 袁润强,E-mail:yuanrunqiang2013@163.com

中图分类号: R737.11

收稿日期: 2016-11-30

Expression and function of OIP5 gene in renal cancer

- 1. Department of Urology, People's Hospital of Zhongshan, Zhongshan, Guangdong, 528403, China;
- 2. Department of Oncology, People's Hospital of Zhongshan;
- 3. Department of Urology, First Affiliated Hospital of Sun Yat-Sen University

More Information

Corresponding author: YUAN Runqiang, E-mail: yuanrunqiang2013@163.com

Received Date: 30 November 2016

摘要

摘要: 目的:探讨OIP5基因与肾癌发生发展的相关性,及下调OIP5基因对肾癌细胞增值活力、侵袭力及成瘤能力的影响。方法:利用RT-PCR及Real-time PCR在4对肾癌组织及癌旁正常组织中检测OIP5的表达情况,利用Real-time PCR在肾正常细胞系HK-2及肾癌细胞系786-O中检测OIP5基因的表达情况。转染shRNA下调OIP5基因后,检测肾癌细胞的增殖活力、侵袭能力及成瘤能力等。进一步利用免疫组化的方法在裸鼠成瘤的组织中检测OIP5蛋白的表达。结果:我们发现OIP5基因在肾癌组织及肾癌细胞系中都明显的高表达。降低OIP5基因的表达后,肾癌细胞的增殖能力、侵袭能力及成瘤能力都明显的减弱了。结论:OIP5基因与肾癌的发生发展有关,OIP5可能是肾癌基因治疗的一个新靶点。
- 肾癌 /
- OIP5基因 /
- 基因治疗
Abstract: Objective:To investigate the relationship between OIP5 gene and the renal cancer risk, and the effect of down regulation of OIP5 gene expression on proliferation, invasion and tumorigenic ability of 786-O cell.Method:We used RT-PCR and real-time PCR to detect the OIP5 expression in four pairs of renal cell carcinoma tissues and adjacent normal tissues.At the same time, we detected OIP5 expression in HK-2and 786-O cell lines.After down regulation of OIP5 gene through transfecting shRNA, we detected proliferation, invasion and tumorigenic ability of 786-O cell.Result:We found OIP5 gene was obviously high expressed in renal cell carcinoma tissues and cell line.After reducing OIP5 gene expression, renal cancer cell proliferation, invasion and tumorigenic ability were significantly attenuate.Conclusion:OIP5gene is associated with the risk of renal cancer, which may be a new target of renal cancer gene therapy.
- renal caner /
- OIP5gene /
- gene therapy

HTML全文

参考文献(16)

[1]	Jemal A, Siegel R, Xu J, et al.Cancer statistics[J].CACancer J Clin, 2010, 60 (5):277-300.
[2]	Gupta K, Miller J D, Li J Z, et al.Epidemiologic and socioeconomic burden of metastatic renal cell carcinoma (mRCC):a literature review[J].Cancer Treat Rev, 2008, 34 (3):193-205.
[3]	Rini B I, Campbell S C, Escudier B.Renal cell carcinoma[J].Lancet, 2009, 373 (9669):1119-1132.
[4]	Weikert S, Ljungberg B.Contemporary epidemiology of renal cell carcinoma:perspectives of primary prevention[J].World J Urol, 2010, 28 (3):247-252.
[5]	Chun H K, Chung K S, Kim H C, et al.OIP5 is a highly expressed potential therapeutic target for colorectal and gastric cancers[J].BMB Rep, 2010, 43 (5):349-354.
[6]	Yazarloo F, Shirkoohi R, Mobasheri M B, et al.Expression analysis of four testis-specific genes AURKC, OIP5, PIWIL2 and TAF7L in acute myeloid leukemia:a gender-dependent expression pattern[J].Med Oncol, 2013, 30 (1):368.
[7]	Koinuma J, Akiyama H, Fujita M, et al.Characterization of an Opa interacting protein 5involved in lung and esophageal carcinogenesis[J].Cancer Sci, 2012, 103 (3):577-586.
[8]	Chung K S, Jang Y J, Kim N S, et al.Rapid screen of human genes for relevance to cancer using fission yeast[J].J Biomol Screen, 2007, 12 (4):568-577.
[9]	Nakamura Y, Tanaka F, Nagahara H, et al.Opa interacting protein 5 (OIP5) is a novel cancer-testis specific gene in gastric cancer[J].Ann Surg Oncol, 2007, 14 (2):885-892.
[10]	Roberts W W, Kavoussi L R.Laparoscopic donor nephrectomy:an upadate[J].Curr Opin Nephrol Hypertens, 2001, 10 (6):771-775.
[11]	Smith R S, Fry W R, Tsoi E K, et al.Gasless laparoscopy and conventional instruments.The next phase of minimally invasive surgery[J].Arch Surg, 1993, 128 (10):1102-1107.
[12]	Flax S.Gasless laparoscopy for the urologist[J].Can JUrol, 1997, 4 (1):309-313.
[13]	Zhai D, Jin C, Huang Z, et al.Differential regulation of Bax and Bak by anti-apoptotic Bcl-2family proteins BclB and Mcl-1[J].J Biol Chem, 2008, 283 (15):9580-9586.
[14]	Ruiz-Vela A, Opferman J T, Cheng E H, et al.Proapoptotic BAX and BAK control multiple initiator caspases[J].EMBO, 2005, 6 (4):379-385.
[15]	Germain M, Milburn J, Duronio V.MCL-1inhibits BAXin the absence of MCL-1/BAX Interaction[J].J Biol Chem, 2008, 283 (10):6384-6392.
[16]	Gong M, Xu Y, Dong W, et al.Expression of Opa interacting protein 5 (OIP5) is associated with tumor stage and prognosis of clear cell renal cell carcinoma[J].Acta Histochem, 2013, 115 (8):810-815.