Effects of insulin resistance on rat chronic prostatitis and the expression of SIgA in prostate tissue
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摘要: 目的：研究大鼠胰岛素抵抗对慢性无菌性前列腺炎发病的影响，并初步探讨其机制。方法：实验分为两组：对照组（CON）、胰岛素抵抗组（IR）。CON组喂养基础饲料，IR组喂养高脂饲料诱导胰岛素抵抗，共喂养10周；糖耐量实验（glucose tolerance test，GTT）和胰岛素耐量实验（insulin tolerance test，ITT）检测大鼠胰岛素抵抗情况；大鼠处死后取前列腺液，通过镜检检测卵磷脂小体密度、红细胞和白细胞；qPCR检测前列腺组织分泌型免疫球蛋白A （Secretory immunoglobulin A，SIgA）的表达水平，Western blot检测前列腺组织SIgA的蛋白表达。结果：IR组大鼠前列腺白细胞数明显高于CON组（P=0.0114），卵磷脂小体密度低于CON组（P=0.0243）；IR组前列腺组织SIgA基因和蛋白表达明显升高。结论：胰岛素抵抗能够明显升高大鼠的前列腺组织炎症反应，可能是慢性无菌性前列腺炎发病的一个高风险因素。Abstract: Objective: To investigate the effects of high-fat diet induced insulin resistance on rat chronic prostatitis (CP) and expression of SIgA.Method: The control (CON) group and insulin resistance (IR) group were established according to the experimental requirements.The IR group was established due to 10-week high-fat diet feed.Glucose and insulin tolerance tests (GTT and ITT) were performed.When two groups of rats were killed, the microscopy was performed to analyze lecithin density、erythrocyte count and leucocyte count.SIgA mRNA levels of prostate tissue were measured by qPCR.Western-blot analysis was used to determine the protein levels of SIgA.Result: Compared with CON group, IR group tended to be insulin resistance obviously.The IR group had higher level of white blood cell and lower level of lecithin bodies.Meanwhile, IR group had higher SIgA mRNA and protein levels.Conclusion: High-fat diet induced insulin resistance, which may be a high risk factors of CP in rat.
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Key words:
- insulin resistance /
- rat /
- chronic prostatitis /
- SIgA
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