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摘要: 目的：探讨前列腺特异性抗原（PSA）、Ki-67在前列腺癌组织中的表达与Gleason评分的相关性。方法：采用免疫组化SP法检测43例前列腺癌患者术后石蜡包埋组织中PSA、Ki-67的表达，并根据苏木精-伊红（HE）切片进行Gleason评分。同时，对患者的术前血总前列腺特异性抗原（tPSA）值和对应HE切片中的组织PSA进行比较。结果：在前列腺癌组织中PSA阳性率为93.0%（40/43），其表达量与Gleason评分呈负相关（r=-0.612，P=0.000）。Ki-67阳性表达率为90.7%（39/43），其表达与Gleason评分呈正相关（r=0.696，P=0.000）。PSA与Ki-67在前列腺癌组织中的表达呈无相关性（r=-0.163，P=0.296）。在术前取患者外周血tPSA与癌组织的PSA相比也呈明显的正相关性（r=0.814，P=0.000）。结论：Gleason评分越高，PSA表达越弱，Ki-67表达越强，前列腺癌组织分化程度越差，预后越差。明确前列腺癌Gleason分级，及检测PSA和Ki-67的表达有利于对患者的预后进行评估。Abstract: Objective: To investigate the correlation between the expression of prostate-specific antigen (PSA), Ki-67 and Gleason score in prostate cancer tissue. Method: The expression of PSA, Ki-67 in 43 cases with prostate cancer after operation were retrospectively assessed through immunohistochemical (IHC) analyses. Gleason score was evaluated according to the HE slices. Result: The positive expression rates of PSA in prostate cancer was 93.0% (40/43), and the expression of PSA and Gleason score was significantly negatively correlated (r=-0.612, P=0.000). The positive expression rates of Ki-67 in prostate cancer was 90.7% (39/43), and it was positively correlated with Gleason score (r=0.696, P=0.000). The expression of PSA and Ki-67 showed no correlation in prostate cancer tissues (r=-0.163, P=0.296). There was also a significant positive correlation between preoperative peripheral blood tPSA and tissue PSA (r=0.814, P=0.000).Conclusion: The higher the Gleason score was, the weaker the expression of PSA was, the stronger the Ki-67 expression was, the worse the degree of differentiation of prostate cancer was. Defining the Gleason score and detecting the expression of PSA and Ki-67 together is helpful to evaluate the prognosis of patients with prostate cancer.
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Key words:
- prostate cancer /
- Gleason score /
- prostate specific antigen /
- Ki-67
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[1] 杨进益, 杨明州, 魏伟, 等.前列腺癌发生发展的流行病学研究进展[J].临床泌尿外科杂志, 2017, 32(9):721-725.
[2] 程亮, 王曦, 朱扬丽.前列腺癌Gleason分级目前进展的剖析[J].中华病理学杂志, 2011, 40(9):577-580.
[3] 朱其聪, 毛高才, 吕扬, 等.前列腺癌组织BSP和PSA的表达及意义[J].肿瘤学杂志, 2014, 20(9):755-757.
[4] 李华镭, 张青, 刘益飞, 等.前列腺癌组织中CXCR7与Ki-67的表达及相关性研究[J].交通医学, 2014, 28(5):445-448.
[5] Koutourousiou M, Georgakoulias N, Kontogeorgos G, et al.Subepen-dymomas of the lateral ventricle:tumor recurrence correlated withincreased Ki-67 labeling index[J].Neurol India, 2009, 57(2):191-193.
[6] 冯小兰, 黄喜健, 农勤高.前列腺癌术后Gleason分级与PSA、P504s、Ki-67蛋白表达相关性分析[J].现代肿瘤医学, 2014, 22(8):1907-1909.
[7] 秦海秋.PSA对前列腺癌诊断的研究[J].中国实验诊断学, 2012, 16(6):1105-1106.
[8] 王海东.PSA、PCNA在前列腺癌中的表达及意义[D].山东:青岛大学, 2002.
[9] 曹亮.免疫组化五项指标(PSA、PAP、HCK、P63、AMACR)的表达在前列腺病变诊断中的应用价值[D].湖南:中南大学, 2010.
[10] Migita T, Oda Y, Naito S.The accumulation of angiostatin-like fragments in human prostate carcinoma[J].Clin Cancer Res, 2001, 7(9):2750-2756.
[11] Jakob C, Liersch T, Meyer W, et al.Predictive value of ki-67 and P53 in locally advanced rectal cancer:correlation with thymidylate synthase and histopathological tumor regression after neoadjuvant 5-fu-based chemoradiotherapy[J].World J Gastroenterol, 2008, 14(7):1060-1066.
[12] 李丹丹, 王迎宾, 李骥.前列腺癌组织中TGF-β1、Ki-67的表达变化及意义[J].山东医药, 2014, 54(31):62-63.
[13] Koutourousiou M, Georgakoulias N, Kontogeorgos G, et al.Subepen-dymomas of the lateral ventricle:tumor recurrence correlated with increased Ki-67 labeling index[J].Neurol India, 2009, 57(2):191-193.
[14] 申丽, 姚敏, 张锦荣, 等.HER-2和Ki-67在前列腺癌中的表达及其与分级和预后的关系[J].现代肿瘤医学, 2013, 21(4):838-840.
[15] 赵朋, 于斌, 刘德祥.Bcl-2、Ki-67在前列腺癌组织中的表达及临床意义[J].天津医药, 2013, 41(10):1022-1023.
[16] Cowen D, Troncoso P, Khoo V S, et al.Ki-67 staining is an independent correlate of biochemical failure in prostate cancer treated with radiotherapy[J].Clin Cancer Res, 2002, 8(5):1148-1154.
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