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摘要: 目的：使用奇异变形杆菌构建大鼠肾脏感染性结石模型。方法：尝试尿道插管将菌液注入膀胱、肾盂穿刺注入菌液及开腹膀胱注射菌液的方法，最后选择膀胱注射法。将34只雄性Wistar大鼠分为四组。实验组根据奇异变形杆菌计数分为106、107和108三组，每组10只大鼠。异氟烷吸入麻醉，取耻骨弓上方下腹部正中切口，切开腹腔，提起膀胱，使用无菌1 ml注射器于膀胱顶部将0.5 ml不同浓度的菌液注入膀胱内，然后退出针头，关闭腹腔，术后6 h恢复进食和饮水。空白对照组4只大鼠，每天普通饲养，膀胱内注入0.5 ml生理盐水。手术后4周以过量麻醉处死所有大鼠。取下腹部正中切口（约3 cm）进入腹腔，将大鼠肾脏、输尿管和膀胱分离。观察脏器大小、表面色泽及结石形成情况，并称重。获取的尿路组织标本置入4%多聚甲醛固定24 h，常规石蜡包埋组织标本。结石标本行红外光谱分析成分。结果：所有大鼠肾脏均未见结石形成。108组中5只大鼠膀胱内有结石形成，成石率为50%。结石成分分析表明结石成分均以六水磷酸镁铵、碳酸磷灰石为主。对照组、106组和107组大鼠膀胱内无结石形成。结论：奇异变形杆菌可在大鼠膀胱中形成感染性结石，而如何有效构建肾脏感染性结石模型有待进一步研究。Abstract: Objective: To establish infection stone in rat bladder using Proteus mirabilis. Method: Thirty-four male Wistar rats were divided into 4 groups. The experimental group was divided into 3 groups according to the burden of Proteus mirabilis(106, 107 and 108). Each experimental group has 10 rats. Under isoflurane inhalation anesthesia and the lower middle abdominal incision was performed, the bladder was showed and lift. A sterile syringe of 1 ml was used to inject different concentrations of bacterial solution in 0.5 ml. For rats in control group, 0.5 ml saline was injected. Each rat ate and drank water after 6-hour recovery. Four weeks after surgery, all rats were sacrificed by overdose anesthesia. The bladder was checked for stones. The urine from bladder was smeared for bacterium identification. The kidney and ureter were also taken. All these organs were fixed in 4% paraformaldehyde for 24 h and then processed for histological observation. Result: There was no renal stone in all rats. In group of 108, bladder stones were found in 5 rats(50%). There was no bladder stones in group 106, 107 or control group. Stone composition analysis showed that the major components were ammonium magnesium phosphate hexahydrate and carbonated apatite.Conclusion: Infection stone can be developed in rat bladder by injection of Proteus mirabilis. Further investigations are needed for establishing the model of kidney infection stone.
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Key words:
- infection stone /
- Proteus mirabilis /
- animal model
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