抗精子抗体对人精子HOS试验及膜间Ca<sup>2+</sup>内流的影响

刘喜军; 李荣军; 王向东; 王璟琦; 徐计秀

doi:10.13201/j.issn.1001-1420.2020.04.009

抗精子抗体对人精子HOS试验及膜间Ca²⁺内流的影响

- 1. 山西医科大学附属大同市第三人民医院泌尿外科(山西大同, 037008);
- 2. 山西医科大学第一医院泌尿外科
基金项目:
山西省卫生健康委科研项目(No:2018117)

详细信息

中图分类号: R698

收稿日期: 2019-07-25

Effects of antisperm antibody on human sperm HOS test and Ca²⁺ influx between membranes

- 1. Department of Urology, Third Hospital of Datong, Datong, Shanxi, 037008, China;
- 2. Department of Urology, First Hospital, Shanxi Medical University

Received Date: 25 July 2019

摘要

摘要: 目的:探讨在正常精液中加入IgG类抗精子抗体阳性精浆对人精子膜功能完整性及压力敏感性Ca²⁺内流的影响。方法:在门诊的不育患者中收集精浆IgG抗精子抗体阳性患者,通过离心的方法从其精液中分离出精浆,并同正常人精子孵育,与正常精浆及正常人精子、精子培养液及正常人精子孵育的对照组比较研究,分别对它们进行低渗肿胀(HOS)试验及测定精子细胞内钙离子浓度的变化。结果:与IgG抗精子抗体阳性精浆孵育的精子,其g形精子百分率和精子尾部总肿胀率均明显低于两个对照组,差异有统计学意义(P<0.01);试验组的精子细胞内Ca²⁺荧光强度差值明显低于两个对照组,差异有统计学意义(P<0.01)。结论:IgG类抗精子抗体对人精子膜功能完整性及压力敏感性Ca²⁺内流均可造成影响。
- 抗精子抗体 /
- 精子 /
- 钙离子 /
- 低渗肿胀试验
Abstract: Objective: To investigate the effect of adding IgG antisperm antibody-positive seminal plasma to normal semen on the functional integrity of human sperm membranes and pressure-sensitive Ca²⁺influx. Method: The seminal plasma IgG antisperm antibody positive semen in infertile patients was collected from the infertile patients in the clinic, and the seminal plasma was separated from the semen by centrifugation and incubated with normal human sperm. And then, the samples were compared with two control groups including both normal semen incubated with normal human sperm and sperm culture fluid incubated with normal human sperm. After that, a hypotonic swelling(HOS) test was performed and the change of calcium ion concentration in sperm cells was measured respectively. Result: Among the sperm incubated with IgG antisperm antibody-positive seminal plasma, the percentage of g-shaped sperm and the total swelling rate of the sperm tail were significantly lower than those of the two control groups, and the difference was significant(P<0.01). The Ca²⁺fluorescence intensity within sperm cells in the test group was also significantly lower than that in the two control groups, and the difference was significant(P<0.01).Conclusion: IgG antisperm antibodies can affect the functional integrity of human sperm membrane and pressure sensitive Ca²⁺influx.
- antisperm antibody /
- sperm /
- calcium ion /
- hypotonic swelling test

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