精浆人附睾分泌蛋白4在精索静脉曲张的表达及其与精子质量的相关性研究

覃天资; 黄敏玉; 黄群

doi:10.13201/j.issn.1001-1420.2020.12.012

精浆人附睾分泌蛋白4在精索静脉曲张的表达及其与精子质量的相关性研究

- 右江民族医学院附属医院泌尿外科(广西百色, 533000)
基金项目:
2018年度广西医药卫生自筹经费科研课题(No:Z20180215)

详细信息

通讯作者: 黄敏玉,E-mail:21161019@qq.com

中图分类号: R698

收稿日期: 2020-03-18

Expression of seminal plasma HE4 in varicocele and its correlation with sperm quality

- Department of Urology, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, China

More Information

Corresponding author: HUANG Minyu, E-mail: 21161019@qq.com

Received Date: 18 March 2020

摘要

摘要: 目的:探讨精浆人附睾分泌蛋白4(HE4)在精索静脉曲张(VC)的表达及其与精子质量的相关性。方法选取2018年1月~2019年6月在我院住院的68例VC男性患者作为观察组,另取25例健康男性为对照组。分别留取精液进行精液质量分析,检测精子密度、精子总活力、前向运动精子水平,同时应用酶联免疫吸附法(ELISA)检测两组精浆中HE4水平。研究精浆HE4在VC的表达及其与精液质量的相关性。将68例VC患者根据精子密度、精子活力及精子形态分为正常精子组、畸形精子组、无精子组、少精子组、弱精子组、少弱畸精子组,比较各组中精浆HE4表达的差异。结果VC患者精子密度、精子总活力、前向运动精子水平均低于对照组,差异均有统计学意义(P＜0.01);VC患者精浆中HE4水平高于对照组,差异有统计学意义(P＜0.01);精浆HE4水平与精子密度、精子总活力、前向运动精子水平呈负相关,差异有统计学意义(P＜0.01)。正常精子组、畸形精子组、无精子组、少精子组、弱精子组、少弱畸精子组中精浆HE4表达差异有统计学意义(P＜0.01),两两比较畸形精子组和少弱畸精子组精浆HE4表达明显高于正常精子组,差异有统计学意义(P＜0.01)。结论VC患者精液质量低于正常男性,与其精浆中HE4的高表达有关,并且VC患者畸形精子数偏高时,精浆中HE4高表达。检测精浆HE4水平能反映VC患者精液质量水平,精浆HE4的高表达能否反映VC的致畸作用值得深入研究,这有助于进一步明确VC导致不育的机制,为临床诊疗及疗效评估提供帮助。
- 人附睾分泌蛋白4 /
- 精索静脉曲张 /
- 精液质量
Abstract: Objective: To investigate the expression of seminal plasma human epididymal secretory protein 4(HE4) in varicocele(VC) and its correlation with sperm quality. Method: From January 2018 to June 2019, 68 male patients with VC hospitalized in our hospital were selected as observation group and 25 healthy men as control group. Semen quality was analyzed, sperm density, total sperm motility and forward motile sperm level were measured, and HE4 levels in seminal plasma of the two groups were detected by enzyme-linked immunosorbent assay(Elisa). The expression of seminal plasma HE4 in VC and its correlation with semen quality were studied. These 68 VC patients were divided into normozoospermia group, asthenoteratozoospermia group, azoospermia group, oligozoospermia group, asthenozoospermia group and oligoasthenoteratozoospermia group according to sperm density, sperm motility and sperm morphology, and the difference of seminal plasma HE4 expression among groups was compared. Result: The sperm density, total sperm motility and forward motile sperm level in patients with VC were significantly lower than those in the control group(P＜0.01), and the level of HE4 in seminal plasma of patients with VC was significantly higher than that in the control group(P＜0.01), and the level of HE4 in seminal plasma was negatively correlated with sperm density, total sperm motility and forward motile sperm level(P＜0.01). Difference in the expression of seminal plasma HE4 in normozoospermia group, asthenoteratozoospermia group, azoospermia group, oligozoospermia group, asthenozoospermia group and oligoasthenoteratozoospermia group was statistically significant(P＜0.01). Expression of seminal plasma HE4 in asthenoteratozoospermia group and oligoteratozoospermia group was significantly higher than that in normozoospermia group(P＜0.01).Conclusion: The semen quality of VC patients is lower than that of normal men, which is related to the high expression of HE4 in seminal plasma. When the number of abnormal sperm in VC patients is on the high level, HE4 is high expression in seminal plasma. The detection of seminal plasma HE4 level can reflect the semen quality level of VC patients. However, whether the high expression of seminal plasma HE4 can respond to the teratogenic effect of VC deserves further study to further clarify the mechanism leading to infertility, and to provide help for clinical diagnosis and treatment and evaluation of curative effect.
- human epididymis secretory protein /
- varicocele /
- semen quality

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