Effect of isoferulic acid on inhibiting cell proliferation and inducing cell apoptosis in human renal carcinoma cell A-498
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摘要: 目的:探究异阿魏酸(isoferulic acid,IFA)抑制人肾癌细胞A-498增殖,诱导细胞凋亡的作用机制。方法:本研究通过克隆形成实验和CCK-8增殖检测实验考察IFA对人肾癌细胞A-498增殖能力的影响;用Annexin V-FITC/PI双染色法检测IFA对A-498细胞凋亡的影响;用免疫印迹法检测IFA对PARP和caspase-3活性的影响,并测定IFA对β-catenin和c-Myc蛋白表达水平的影响。结果:IFA显著抑制人肾癌细胞A-498细胞克隆的增殖活性,促进A-498细胞凋亡,并诱导cleaved PARP和cleaved caspase-3的表达;同时,IFA可以剂量依赖地下调β-catenin和c-Myc的表达,诱导细胞凋亡。结论:IFA下调β-catenin和c-Myc的表达,诱导A-498细胞凋亡,抑制A-498细胞增殖,是治疗肾癌的潜在候选化合物。Abstract: Objective: To investigate the effect of isoferulic acid(IFA) on the cell proliferation and apoptosis of human renal carcinoma cell A-498.Methods: The effect of IFA on the cell proliferation of A-498 cells was detected by colony formation assay and cell counting kit-8 assay. Annexin V-FITC/PI double staining was used to detect the effect of IFA on the apoptosis of A-498 cells. Western blotting was used to detect PARP and caspase-3 activity as well as the expression of β-catenin and c-Myc.Results: IFA dose-dependently inhibited the proliferation of A-498 cells. IFA induced the apoptosis of A-498 cells and the expression of cleaved PARP and cleaved caspase-3 were increased upon treatment of IFA. In addition, IFA downregulated the expression of β-catenin and c-Myc in a dose-dependent manner.Conclusion: Our findings suggested that IFA downregulates the expression of β-catenin and c-Myc, promotes the apoptosis of human renal carcinoma cell A-498 and inhibits the proliferation of A-498 cells. IFA could be a potential candidate for the treatment of renal carcinoma.
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Key words:
- isoferulic acid /
- human renal cell carcinoma /
- apoptosis /
- c-Myc
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