Expression characteristics of LncRNA-MEG3 in different parts of congenital ureteropelvic junction stenosis
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摘要: 目的 检测长非编码RNA母系表达基因3(long non coding RNA-maternally expressed gene 3，LncRNA MEG3)在由肾盂输尿管连接部(ureteropelvic junction，UPJ)狭窄所致先天性肾盂输尿管连接部梗阻(ureteropelvic junction obstruction，UPJO)经Anderson-Hynes术治疗后在患儿的不同解剖部位组织中的表达，并探讨与术后肾功能改善率的相关性，及其在该病发生中的作用及机制。方法 收集2020年5月—2022年5月20例患儿在新疆医科大学第一附属医院接受诊疗行Anderson-Hynes术，将术中切除的UPJ组织作为研究对象，标本离体后立即按解剖部位分为正常对照组织肾盂段(renal pelvis，RP)组20例、UPJ组20例、远端输尿管狭窄组(distal ureterostenosis，DU)组20例，通过Masson三色染色、免疫组织化学(immunohistochemistry，IHC)、实时荧光定量PCR(q-PCR)的手段观察3组标本胶原容积分数(collagen volume fraction，CVF)、Cajal间质细胞(interstitial cells of cajal，ICCs)数目、LncRNA-MEG3表达，并分析LncRNA-MEG3表达、ICCs数目与患儿术后半年分肾功能(split renal function，SRF)改善率的相关性。结果 CVF在UPJ组最高，明显高于RP组和DU组，差异有统计学意义(P < 0.05)。LncRNA-MEG3在UPJ组高表达，RP组、UPJ组、DU组中MEG3的表达量分别为0.860±0.205、2.160±0.321、0.838±0.325，差异有统计学意义(P < 0.001)。术后半年分肾功能改善率与UPJ中LncRNA-MEG3的表达量、ICCs数目均不相关。结论 UPJO患儿UPJ部ICCs的数目比肾盂段、远端输尿管狭窄段少。UPJ组胶原纤维增生程度比肾盂段、远端输尿管狭窄段重；LncRNA-MEG3在UPJ组高表达，可为探索先天性UPJ狭窄的病因机制及寻找靶基因提供参考；UPJ中LncRNA-MEG3的表达量、ICCs数目与术后半年分肾功能改善率均不相关。
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关键词:
- 肾积水 /
- 肾盂输尿管连接部狭窄 /
- Cajal间质细胞 /
- 长非编码RNA母系表达基因3
Abstract: Objective Long non-coding RNA-maternally expressed gene 3 (LncRNA-MEG3) in different anatomical tissues of children with congenital ureteropelvic junction obstruction (UPJO) after Anderson-Hynes surgery were detected. The correlation between the rate of renal function recovery and its role and mechanism in the occurrence of the disease were explored.Methods Tissues of ureteropelvic junction resected during Anderson-Hynes surgery from twenty cases in First Affiliated Hospital of Xinjiang Medical University were collected as the research objects. The specimens were divided into normal control tissues (renal pelvis, RP group, n=20), ureteropelvic junction tissues (UPJ group, n=20) and distal ureter tissues (distal ureterostenosis, DU group, n=20) according to the anatomical location. Masson trichrome staining, immunohistochemistry (IHC), and real-time fluorescence quantitative PCR (q-PCR) were used to observe the collagen volume fraction (CVF) in the three groups. The number of interstitial cells of Cajal (ICCs), the expression of LncRNA-MEG3, and the correlation between the expression of LncRNA-MEG3 and the number of ICCs and the recovery rate of split renal function at half a year after operation were analyzed.Results The CVF was the highest in UPJ group, which was significantly higher than that in RP group and DU group (P < 0.05). LncRNA-MEG3 was highly expressed in UPJ group. The expression of MEG3 in RP group, UPJ group and DU group were (0.860±0.205), (2.160±0.321), (0.838±0.325), respectively, and the difference was statistically significant (P < 0.001). The recovery rate of split renal function at half a year after surgery was not correlated with the expression of LncRNA-MEG3 or the number of ICCs in the ureteropelvic junction.Conclusion The number of ICCs in UPJ is less than that in renal pelvis and distal ureterostenosis in children with UPJO. The degree of collagen fiber hyperplasia in UPJ group was more severe than that in renal pelvis and distal ureterostenosis segment. LncRNA-MEG3 is highly expressed in UPJ group, which may provide reference for exploring the etiology and target genes of congenital UPJ stenosis. The expression of LncRNA-MEG3 and the number of ICCs in the ureteropelvic junction were not correlated with the recovery rate of split renal function at half a year after surgery. -
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表 1 q-PCR中使用基因序列
基因 序列 hu-LcnRNA-MEG3-F GGGAAGGGACCTCGAATGTG hu-LcnRNA-MEG3-R GTGGGAATAGGTGCAGGGTG hu-ACTB-F CATGTACGTTGCTATCCAGGC hu-ACTB-R CTCCTTAATGTCACGCACGAT 
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表 2 不同组间ICCs数目和CVF比较
组间对比 数值 P值 ICCs RP vs UPJ 23.000±4.729 4.000±2.496 0.01 RP vs DU 23.000±4.729 6.000±0.431 0.01 UPJ vs DU 4.000±2.496 6.000±0.431 0.07 CVF RP vs UPJ 0.410±0.131 0.775±0.110 0.01 RP vs DU 0.410±0.131 0.270±0.079 0.01 UPJ vs DU 0.775±0.110 0.270±0.079 0.03
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