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摘要: 目的：探讨E2F1在肾透明细胞癌中的表达及意义。方法：通过实时定量PCR和Western blot方法检测E2F1在肾透明细胞癌及对应瘤旁组织中mRNA和蛋白的表达情况，并分析E2F1的mRNA水平与临床病理资料的关系及表达相关性。结果：与对应瘤旁组织相比，E2F1的mRNA水平在肾透明细胞癌手术标本中表达明显升高（P=0.0002），相应的蛋白水平对比与mRNA变化一致；E2F1的mRNA水平在不同年龄、性别组间差异无统计学意义（P>0.05），而在组织学分级、肿瘤直径大小、T分期、临床分期和大血管浸润与否差异有统计学意义（P<0.05）。结论：E2F1表达上调可能在肾透明细胞癌的肿瘤形成中发挥作用，且E2F1表达升高可能促进肾透明细胞癌的恶性进展。Abstract: Objective: To investigate the E2F1 expression and significance in clear cell renal cell carcinoma(ccRCC). Methods: Message RNA and protein levels of E2F1 in 52 cases of ccRCC surgical specimens and their corresponding adjacent normal renal tissue samples were detected by real-time PCR and Western blot respectively, the relationship between the mRNA levels of E2F1 and clinicopathological features were analyzed.Results: The mRNA levels of E2F1 were significantly up-regulated in ccRCC tissues compared to adjacent normal renal tissues (P=0.0002).The tendency of the protein levels alteration in ccRCC and Peritumoral tissues were correspond with that of the mRNA levels. The mRNA levels of E2F1 distinguished largely among different groups in terms of histological staging, tumor size, T staging, clinical stages and vascular infiltration (P<0.05), but not age and sex (P>0.05).Conclusions: E2F1 may be a key event in the carcinogenesis and development of ccRCC.
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Key words:
- clear cell renal cell carcinoma /
- E2F1 /
- malignant progression
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