The effect of mesenchymal stem cells on angiogenesis of prostate cancer cell line RM-1 in vivo
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摘要: 目的：探讨间充质干细胞（marrow mesenchymal stem cells，MSCs）对RM-1小鼠前列腺癌细胞生长的影响及其可能机制。方法：从小鼠骨髓中无菌分离培养扩增MSCs。将MSCs与RM-1细胞混合后接种于Balb/c小鼠皮下，观察肿瘤生长情况；采用免疫组化法检测肿瘤组织血管生成情况（CD34染色）；采用Realtime PCR法和ELISA法检测MSCs促进血管生成相关分子的表达情况。结果：MSCs与RM-1小鼠前列腺癌细胞混合后接种于Balb/c小鼠皮下，与对照组相比，MSCs可明显促进肿瘤生长（P<0.05）；前列腺癌组织中CD34免疫组化染色结果证实MSCs组较对照组微血管密度显著升高（P<0.05）；Realtime PCR及ELISA结果显示MSCs条件培养上清中VEGF表达显著升高。结论：MSCs可能通过表达VEGF促进前列腺癌血管形成，从而达到促进肿瘤生长的目的。Abstract: Objective: To investigate the effect of mesenchymal stem cells(MSCs) on angiogenesis of prostate cancer cell line RM-1 in vivo.Methods: MSCs were isolated and culture-expanded from mouse bone marrow. Prostate cancer cell line RM-1 cells were mixed with MSCs and subcutaneous plated in Balb/c mice to observe the tumor growth in vivo. CD34 immunohistochemistry staining were used to examine the micro-vessel density(MVD) in tumor tissue. Realtime PCR and ELISA assay were employed to detect the expression of proangiogenesis factors in MSCs.Results: Compared with control groups, MSCs could effectively promote the growth of RM-1 cells in vivo (P<0.05). The result from immunohistochemistry demonstrated that the MVD of prostate cance tissue is significantly higher in MSCs group than control group. Realtime PCR showed that there existing VEGF expression in MSCs and the result from ELISA assay confirmed that VEGF could be detected in the condition medium of MSCs.Conclusions: MSCs may promote the growth of prostate cancer cell line RM-1 in vivo by stimulate angiogenesis via production of VEGF.
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