曲古抑菌素A膀胱灌注对大鼠膀胱癌生长的抑制作用

李功成; 潘铁军; 田雨冬; 叶章群

曲古抑菌素A膀胱灌注对大鼠膀胱癌生长的抑制作用

- 1. 广州军区武汉总医院泌尿外科(武汉, 430070);
- 2. 郑州市人民医院泌尿外科;
- 3. 华中科技大学同济医学院附属同济医院泌尿外科

详细信息

通讯作者: 潘铁军,E-mail:mwkptj@yahoo.com.cn

中图分类号: R737.14

收稿日期: 2012-07-21

Inhibitory effect of intravesical instillation of trichostatin A in rats with bladder cancer

- 1. Department of Urology, Wuhan General Hospital of Guangzhou Command, Wuhan, 430070, China;
- 2. Department of Urology, the People's Hospital of Zhengzhou;
- 3. Department of Urology, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology

More Information

Corresponding author: PAN Tiejun, E-mail: mwkptj@yahoo.com.cn

Received Date: 21 July 2012

摘要

摘要: 目的：探讨曲古抑菌素A（TSA）膀胱灌注对大鼠膀胱肿瘤生长的抑制作用和机制。方法：将42只雌性Wistar大鼠，随机分为对照组和TSA组，制作MNU诱导大鼠原位膀胱癌模型，在第10周分别行生理盐水和TSA连续膀胱灌注，每周1次，共7次。分别在灌注的第l2周和17周进行病理观察、测量膀胱总重量、凋亡指数及western blot检测。结果：与对照组相比，TSA组大鼠膀胱肿瘤小且单发较多。TSA组大鼠膀胱的重量小于对照组（P<0.05）；TSA组大鼠膀胱肿瘤的凋亡指数大于对照组（P<0.01），TSA组大鼠膀胱肿瘤组织XIAP的表达较对照组明显降低。结论：TSA膀胱灌注可抑制大鼠膀胱肿瘤生长，延缓肿瘤进展，其机制与诱导肿瘤细胞凋亡有关，并可能涉及相关基因XIAP表达的调控。
- 膀胱癌 /
- 曲古抑菌素A /
- 大鼠 /
- 凋亡
Abstract: Objective: To investigate the effect and mechanism of intravesical instillation of trichostatin A in bladder cancer of rats. Method: Forty-two fema1e Wistar rats were randomized into control group and trichostatin A group. Orthotopic bladder tumors were established in rats by intravesical administration of N-mmethyl-noitrosourea. Rats of control and trichostatin A group received a total of seven times(every week) intravesical instillation of physiologic saline and trichostatin A respectively from the tenth week.Rats were executed in the twelfth and seventeenth week and their weights of bladder,pathologic characters,apoptotic indexes were surveyed.The expression of XIAP protein was detected by Western blot. Result: Compared with control group, the bladder tumors of trichostatin A group were small and more single. The bladder weights of trichostatin A group were significantly lower than those in control group(P<0.05), but the apoptotic indexes of control group were significantly lower than those in trichostatin A group(P<0.01). It was showed by Western blot that the XIAP protein level dropped after Intravesical instillation of trichostatin A. Conclusion: Intravesical instillation of trichostatin A can inhibit the growth and progression of bladder tumour by inducing apoptosis in rats with bladder cancer, which might be related to the expression of XIAP.
- bladder cancer /
- trichostatin A /
- rat /
- apoptosis

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