Expression of circadian clock protein Rev-erbα in prostate tissues of different degrees of inflammation
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摘要: 目的 探讨良性前列腺增生(BPH)人群中前列腺组织炎症与生物钟蛋白Rev-erbα表达的相关性。方法 收集诊断为BPH并行经尿道前列腺手术的前列腺组织标本。对前列腺组织进行炎症程度分级。免疫组化(IHC)评估前列腺组织中CD4、CD8、白细胞介素(IL)-6、IL-8、IL-1β和Rev-erbα的表达情况；qRT-PCR检测前列腺组织中IL-1β、IL-6和IL-8和Rev-erbα的表达水平。分析前列腺组织炎症程度与前列腺组织Rev-erbα表达强度的相关性。结果 收集84例BPH组织标本，73例(86.9%)伴有不同程度的组织炎症。IHC提示前列腺组织中不同炎症程度与炎症因子(IL-6、IL-8和IL-1β)表达强度一致；组织炎症细胞中，CD4+细胞数量远多于CD8+细胞数量；前列腺组织中Rev-erbα的表达强度与前列腺组织炎症程度呈负相关，其相关系数为-0.691(P < 0.001)。qRT-PCR提示不同的前列腺组织炎症程度与组织中IL-1β、IL-6、IL-8的mRNA表达差异趋势一致，与组织中Rev-erbα的mRNA表达差异趋势相反。结论 生物钟蛋白Rev-erbα可能在前列腺组织中有降低组织局部炎症的作用，可能成为防治前列腺组织炎症的潜在靶点。
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关键词:
- 良性前列腺增生 /
- 组织炎症 /
- 生物钟蛋白Rev-erbα
Abstract: Objective To investigate the relationship between inflammation of prostate tissue and the expression of clock protein Rev-erbα in prostatic tissues.Methods Prostate tissue specimens diagnosed as BPH and undergoing transurethral prostate surgery were collected. The inflammation degree of prostate tissue was graded. Immunohistochemistry(IHC) was used to evaluate the expression of CD4, CD8, IL-6, IL-8, IL-1β and Rev-erbα in prostate tissue, and the expression level of IL-1β, IL-6, IL-8 and Rev-erbα were detected by qRT-PCR in prostate tissues. The correlation between the degree of inflammation in prostate tissue and the expression intensity of Rev-erbα in prostate tissue was analyzed.Results A total of 84 cases of prostate tissue specimens were collected, of which 73 cases(86.9%) were accompanied by tissue inflammation. IHC showed that different levels of inflammation in prostate tissue were consistent with the expression intensity of inflammatory factors(IL-6, IL-8 and IL-1β), and the number of CD4+ cells was far greater than the number of CD8+ cells. The expression intensity of Rev-erbα in prostate tissue was negatively correlated with the degree inflammation in prostate tissues, and the correlation coefficient was-0.691(P < 0.001). QRT-PCR indicated the trend of differences in the expression of IL-1β, IL-6 and IL-8 was consistent with the trend of the degree of inflammation in prostate tissues, but the trend of differences in the expression of Rev-erbα was opposite to the trend of the degree of inflammation in prostate tissues.Conclusion The circadian clock protein Rev-erbα may have the effect of reducing local inflammation in the prostate tissue, and it may become a potential target for the prevention and treatment of inflammation in the prostate tissue. -
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表 1 前列腺组织Rev-erbα的表达强度与组织炎症程度相关性分析
生物钟蛋白 表达强度 前列腺组织炎症程度/例 相关性 无(11例) 轻度(30例) 中度(31例) 重度(12例) r P Rev-erbα 轻度表达 0 3 12 11 -0.691 < 0.001 中度表达 0 14 17 1 高度表达 11 13 2 0 
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