Effects of kaempferol on proliferation and metastasis of prostate cancer 22RV1 cells based on PI3K/AKT/mTOR axis
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摘要: 目的 观察山柰酚对人前列腺癌22RV1细胞增殖及转移的影响，并探讨可能机制。方法 取对数期22RV1细胞，随机分为对照组、山柰酚组、胰岛素样生长因子[磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白(mTOR)信号轴激活剂IGF-1]组、山柰酚联合IGF-1组。对照组常规培养，山柰酚组加入山柰酚(80 μmol/L)，IGF-1组加入IGF-1(100 μg/L)，山柰酚联合IGF-1组加入山柰酚(80 μmol/L)及IGF-1(100 μg/L)。MTT法检测细胞增殖能力；伤口愈合实验检测细胞迁移能力；Transwell实验检测细胞侵袭能力；Western blot法检测细胞Ki67、AKT、p-AKT、mTOR、p-mTOR蛋白表达量。结果 与对照组比较，山柰酚组24、48、72 h吸光度值，迁移率，Ki67蛋白表达量，p-AKT/AKT、p-mTOR/mTOR降低，侵袭细胞数减少(P＜0.05)，IGF-1组24、48、72 h吸光度值，迁移率，Ki67蛋白表达量，p-AKT/AKT、p-mTOR/mTOR升高，侵袭细胞数增加(P＜0.05)；与山柰酚组比较，山柰酚联合IGF-1组24、48、72 h吸光度值，迁移率，Ki67蛋白表达量，p-AKT/AKT、p-mTOR/mTOR升高，侵袭细胞数增加(P＜0.05)；与IGF-1组比较，山柰酚联合IGF-1组24、48、72 h吸光度值，迁移率，Ki67蛋白表达量，p-AKT/AKT、p-mTOR/mTOR降低，侵袭细胞数减少(P＜0.05)。结论 山柰酚可抑制前列腺癌22RV1细胞增殖、迁移及侵袭，抑制PI3K/AKT/mTOR信号轴可能是其作用机制之一。Abstract: Objective To observe the effect of kaempferol on the proliferation and metastasis of human prostate cancer 22RV1 cells, and to explore the possible mechanism.Methods Log phase 22RV1 cells were taken and randomly divided into control group, kaempferol group, insulin-like growth factor[phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin](mTOR) signaling axis activator IGF-1]group, kaempferol combined with IGF-1 group. The control group was routinely cultured, the kaempferol group was added with kaempferol (80 μmol/L), the IGF-1 group was added with IGF-1 (100 μg/L), and the kaempferol combined with IGF-1 group was added with kaempferol (80 μmol/L) and IGF-1 (100 μg/L). Cell proliferation was detected by MTT assay. Wound-healing assays were tested for cell migration ability. Cell invasion ability was detected by Transwell assay. Western blot was used to detect the protein expressions of Ki67, AKT, p-AKT, mTOR and p-mTOR.Results Compared with the control group, the absorbance value at 24, 48, and 72 h, migration rate, Ki67 protein expression, p-AKT/AKT, p-mTOR/mTOR and the number of invasive cells were decreased in the kaempferol group (P < 0.05), while the absorbance value at 24, 48, and 72 h, migration rate, Ki67 protein expression, p-AKT/AKT, p-mTOR/mTOR and the number of invasive cells were increased in the IGF-1 group (P < 0.05). Compared with the kaempferol group, the absorbance value at 24, 48, and 72 h, migration rate, Ki67 protein expression, p-AKT/AKT, p-mTOR/mTOR and the number of invasive cells were increased in the kaempferol combined with IGF-1 group (P < 0.05). Compared with the IGF-1 group, the absorbance value at 24, 48, and 72 h, migration rate, Ki67 protein expression, p-AKT/AKT, p-mTOR/mTOR and the number of invasive cells were decreased in the kaempferol combined with IGF-1 group (P < 0.05).Conclusion Kaempferol can inhibit the proliferation, migration and invasion of prostate cancer 22RV1 cells, and inhibition of PI3K/AKT/mTOR signaling axis may be one of its mechanisms.
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Key words:
- kaempferol /
- prostate cancer /
- proliferation /
- metastasis /
- phosphatidylinositol 3-kinase /
- protein kinase B
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表 1 各组22RV1细胞MTT吸光度值比较
X±S 组别 24 h 48 h 72 h 对照组 0.31±0.03 0.55±0.04 0.69±0.05 山柰酚组 0.16±0.011) 0.23±0.021) 0.40±0.021) IGF-1组 0.56±0.021) 0.78±0.061) 0.91±0.071) 山柰酚联合IGF-1组 0.21±0.012)3) 0.42±0.012)3) 0.54±0.042)3) 与对照组比较，1)P＜0.05；与山柰酚组比较，2)P＜0.05；与IGF-1组比较，3)P＜0.05。 
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表 2 各组22RV1细胞Ki67蛋白表达量比较
X±S 组别 Ki67 对照组 0.49±0.04 山柰酚组 0.17±0.011) IGF-1组 1.12±0.071) 山柰酚联合IGF-1组 0.32±0.022)3) 与对照组比较，1)P＜0.05；与山柰酚组比较，2)P＜0.05；与IGF-1组比较，3)P＜0.05。
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表 3 各组22RV1细胞迁移率比较
X±S 组别 迁移率/% 对照组 72.88±4.52 山柰酚组 14.24±0.721) IGF-1组 94.52±6.061) 山柰酚联合IGF-1组 35.28±2.022)3) 与对照组比较，1)P＜0.05；与山柰酚组比较，2)P＜0.05；与IGF-1组比较，3)P＜0.05。
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表 4 各组侵袭细胞数比较
X±S 组别 侵袭细胞/个 对照组 273.02±19.27 山柰酚组 42.59±1.221) IGF-1组 472.85±20.481) 山柰酚联合IGF-1组 187.24±10.782)3) 与对照组比较，1)P＜0.05；与山柰酚组比较，2)P＜0.05；与IGF-1组比较，3)P＜0.05。
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表 5 各组22RV1细胞Ki67蛋白表达量比较
X±S 组别 p-AKT/AKT p-mTOR/mTOR 对照组 0.37±0.03 0.41±0.02 山柰酚组 0.11±0.011) 0.14±0.011) IGF-1组 0.48±0.021) 0.72±0.031) 山柰酚联合IGF-1组 0.21±0.012)3) 0.29±0.022)3) 与对照组比较，1)P＜0.05；与山柰酚组比较，2)P＜0.05；与IGF-1组比较，3)P＜0.05。
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