Expression and significance of Livin and HIF-1α protein in carcinoma of urinary bladder
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摘要: 目的：探讨凋亡抑制蛋白Livin和缺氧诱导因子-1α（HIF-1α）在膀胱尿路上皮癌组织中的表达及其与膀胱尿路上皮癌复发的关系。方法：应用免疫组化SABC法检测45例膀胱尿路上皮癌及15例正常膀胱黏膜组织石蜡切片中Livin和HIF-1α的表达情况，并结合临床资料进行分析。结果：45例膀胱尿路上皮癌组织中分别有23（51.1%）例Livin阳性和39（86.7%）例HIF-1α阳性。在正常组织中有1（6.7%）例Livin阳性，3（20.0%）例HIF-1α阳性。二者在膀胱尿路上皮癌组织中的表达均明显高于相应正常膀胱组织（P<0.01）。Livin蛋白和肿瘤复发成正相关，病理分级之间存在差异；但Livin和HIF-1α的表达均与膀胱尿路上皮癌的临床分期和患者年龄无关（P>0.05）；此外，HIF-1α在病理分级上也无显著差异；Livin与HIF-1α之间也不存在相关性。结论：检测Livin和HIF-1α蛋白有助于评估肿瘤的生物学行为，并有利于膀胱尿路上皮癌患者的临床治疗决策。Abstract: Objective: To investigate the expression of the inhibitor of apoptosis protein Livin and HIF-1alpha/HIF-1α in human carcinoma of urinary bladder and their relationship with cancer development and relapse.Method: The expression of Livin and HIF-1α protein in 45 carcinoma of urinary bladder specimens and 15 corresponding normal mucosa tissues were detected by immunohistochemieal staining.Result: Livin and HIF-1 αprotein positive expression rate in tumor samples were 51.1%(23/45),86.7%(39/45), respectively. While Livin and HIF-1αprotein positive expression rate in corresponding normal mucosa tissues samples were 6.7%(1/45), 20.0%(3/15), respectively. The expression of Livin and HIF-1α protein in the carcinoma of urinary bladder were significantly higher than normal tissue (P<0.01). Tumor recurrence and tumor stage were found with expression of Livin. But no correlation was found between the expression of Livin and HIF-1α in tumor grade and age(P>0.05). There was no significant correlation between the expression of Livin and HIF-1α.Conclusion: The determination of Livin and HIF-1α will be useful in predicting tumor biological behavior and may help for clinical decision-making in the treatment of carcinoma of urinary bladder patients.
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Key words:
- carcinoma of urinary bladder /
- Livin /
- HIF-1α /
- immunohistochemistry
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