荧光原位杂交技术在膀胱尿路上皮癌的诊断和复发中的应用

刘亚东; 顾沈阳; 王业华; 吴蔚

荧光原位杂交技术在膀胱尿路上皮癌的诊断和复发中的应用

- 苏北人民医院泌尿外科(江苏扬州, 225001)

详细信息

通讯作者: 顾沈阳,E-mail:billups0123@163.com

中图分类号: R737.14

收稿日期: 2012-05-11

Application of fluorescent in situ hybridization in the diagnosis and recurrence of bladder cancer

- Department of Urology, Northern Jiangsu People's Hospital, Yangzhou, Jiangsu, 225001, China

More Information

Corresponding author: GU Shenyang, E-mail: billups0123@163.com

Received Date: 11 May 2012

摘要

摘要: 目的：探讨荧光原位杂交技术（fluorescent in situ hybridazation，FISH）在膀胱尿路上皮肿瘤的诊断和术后复发中的应用。方法：选取2008年8月~2011年12月67例膀胱肿瘤患者，包括10例非尿路上皮癌患者和57例膀胱尿路上癌患者，留取受检者的晨尿，做FISH检测。结果：FISH检测初发膀胱肿瘤患者的敏感性是81.3%，FISH检测复发膀胱肿瘤的敏感性是88.9%，FISH检测初发膀胱肿瘤和复发膀胱肿瘤的敏感性差异无统计学意义。随着肿瘤分级的增高，FISH检测的总敏感性逐渐增高（G₁ 79.2%、G₂ 81.0%、G₃ 91.7%），但中低级肿瘤与高级别肿瘤的敏感性差异无统计学意义（χ²=0.267，P=0.605>0.05）。FISH在检测肿瘤个数为1个、2~7个、>7个时的总敏感性分别是80.9%、88.9%、100%。FISH在检测肿瘤直径<3 cm、≥ 3 cm时总的敏感性分别是80.0%、91.7%。结论：FISH在检测初发和复发膀胱肿瘤的敏感性均较高，所以FISH技术作为诊断和检测肿瘤复发的手段，能明显提高检测肿瘤的效率。
- 膀胱肿瘤 /
- 荧光原位杂交技术(FISH) /
- 肿瘤复发 /
- 敏感性
Abstract: Objective: To explore the application of fluorescent in situ hybridization(FISH)in the diagnosis and recurrence of urothelial tumor of bladder.Method: From August 2008 to December 2011,67 volunteers with informed consent were divided into three groups. In these groups,10 none- urothelial tumor of bladder individuals as control,48 patients were proven as urothlial tumor of bladder by pathological,and 9 patients were proven as recurrence of bladder tumor by pathological. Urine were collected and tested by FISH.Result: The sensitivity of FISH was 81.3% in initial bladder cancer and that of the recurrence bladder cancer was 88.9%.while The sensitivity of FISH in different grades(G₁, G₂, G₃)was 79.2%、81.0%、91.7%.Overall sensitivity of bladder carcinoma between G₁, G₂ and G₃ hasn't significant difference. The sensitivity of FISH in detecting the different number of tumors(1, 2-7, >7)was 80.9%、88.9%、100.0%.The sensitivity of FISH in detecting different diameters(<3 cm、≥ 3 cm)was 80.0%、91.7%.Conclusion: FISH as a newly non-invasive technique has a high sensitivity in initial and recurrence bladder cancer. FISH may play an important role in improving the rate of bladder cancer.
- bladder tumor /
- fluorescent in situ hybridization(FISH) /
- tumor recurrence /
- sensitivity

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