OCTN2在1例正常生育男性附睾中的表达报告

李东; 冯自卫; 毕学成; 冼志勇; 叶楚津; 罗耀雄; 王怀鹏; 郑祥光; 刘久敏

OCTN2在1例正常生育男性附睾中的表达报告

- 广东省人民医院泌尿外科(广州, 510080)
基金项目:
广东省科技计划基金资助项目（编号2010B080701090）

详细信息

通讯作者: 冯自卫,E-mail:endourologic@126.com

中图分类号: R69

收稿日期: 2013-03-03

Expression of OCTN2 in human epididymis and its significance

- Department of Urology, Guangdong Academy of Medical Science, Guangdong General Hospital, Guangzhou, 510080, China

More Information

Corresponding author: FENG Ziwei, E-mail: endourologic@126.com

Received Date: 03 March 2013

摘要

摘要: 目的：研究人类附睾有机阳离子转运子2（OCTN2）的mRNA表达特征及蛋白表达特征，为进一步探讨附睾肉碱转运机制提供理论依据。方法：采用RT-PCR方法检测人类附睾组织头部、体部及尾部OCTN2基因的表达；并用Westernblot方法检测该基因在附睾中的蛋白表达，计算其在蛋白水平的相对表达量。结果：OCTN2 mRNA在人附睾头、体、尾组织中均有OCTN2表达，表现为附睾头部表达较弱，而附睾体、尾部分表达丰富；OCTN2蛋白在人附睾头部表达较弱，表达量为（0.71±0.09），附睾体、尾部分表达较丰富，表达量分别为（0.95±0.22）与（0.99±0.15）。结论：两种方法均证实有机阳离子转运子2在人类附睾中有表达，且呈现附睾头部表达较弱，附睾体、尾部分表达丰富的特征，为进一步研究附睾肉碱转运机制奠定基础。
- 人类附睾 /
- OCTN2 /
- 蛋白表达 /
- westernblot
Abstract: Objective: To study the expression of OCTN2 mRNA and protein in human epididymis so as to provide a further theoretical basis for the mechanism of L-Carnitine transport.Method: We collected epididymides from a prostate cancer patient and determined the expressions of OCTN2 mRNA and it's protein in the caput,corpus and cauda of the epididymis by RT-PCR and Westernblot and calculated the relative content of the protein.Result: OCTN2 Mrna was expressed in the caput,corpus and cauda of the epididymis and the expression level in the corpus and cauda of the epididymis were higher than in the caput.OCTN2 protein was also expressed in human epididymis and the relative content was (0.71±0.09) in the caput of the epididymis.It was lower than the relative content (0.95±0.22) and (0.99±0.15) respectively in the corpus and cauda of the epididymis.Conclusion: The expression of OCTN2 mRNA and protein in human epididymis was certificated by RT-PCR and westernblot. They both indicated that the expression level was low in the caput of the epididymis and the expression level was high in the corpus and cauda of the epididymis.This conclusion will play an important role in the study of the mechanism of L-Carnitine transport.
- human epididymis /
- OCTN2 /
- protein expression /
- westrnblot

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