多中心尿路上皮肿瘤克隆起源的FISH研究

王官峰; 李泉林

多中心尿路上皮肿瘤克隆起源的FISH研究

王官峰¹,
李泉林^2, ,

- 1. 3201医院泌尿外科(陕西汉中, 723000);
- 2. 大连医科大学附属第一医院泌尿外科

详细信息

通讯作者: 李泉林,E-mail:qlli2002@yahoo.com.cn

中图分类号: R737.1

收稿日期: 2012-10-08

The clonal origin of multifocal urothelial carcinoma studied by fluorescence in situ hybridization

WANG Guanfeng¹,
LI Quanlin^2, ,

- 1. Department of Urology, 3201 Hospital, Hanzhong, Shanxi, 723000, China;
- 2. Department of Urology, First Affiliated Hospital, Dalian Medical University

More Information

Corresponding author: LI Quanlin, E-mail: qlli2002@yahoo.com.cn

Received Date: 08 October 2012

摘要

摘要: 目的：探讨多中心尿路上皮肿瘤的克隆起源。方法：通过荧光原位杂交（fluorescence in situ hybridization，FISH）技术对28例患者70枚肿瘤的3、7、9、17号染色体的变异类型进行检测，判断多中心灶间染色体变异类型是否一致。结果：28例患者中，26例（92.9%）被认为来自相同的克隆起源，2例来自不同的克隆起源。2例独立克隆起源的患者均为异时性肿瘤，且原发肿瘤与随后发生的肿瘤间隔时间较久，分别约为5年和11年。结论：大多数多中心尿路上皮肿瘤有相同的克隆起源，为单克隆起源可能，且多为同时性肿瘤。多克隆起源多见于异时性肿瘤，时间间隔越久，独立克隆起源的可能性越大。
- 尿路上皮癌 /
- 荧光原位杂交 /
- 克隆 /
- 多发 /
- 复发
Abstract: Objective: To investigate the clonal origin of multifocal urothelial carcinoma.Method: There were 28 cases of multifocal urothelial carcinoma with total of 70 tumors included in the study. Twenty-one of them were simultaneous urothelial carcinoma and 9 were metachronous. Two cases have both simultaneous tumor and metachronous tumor. Aberration of urothelial carcinoma with high frequency at 3, 7, 9, 17 chromosomes were selected and detected by using CSP3/CSP7 DNA probe combinations and GLP p16/CSP17 DNA probe combinations. Chromosomal aberrations type was analyzed and compared to determine the clonality of multifocal tumors.Result: Of the 28 cases, 26 cases(92.9%) shared concordant type of chromosomal aberrations, and considered of common clonal origin, 2 cases were detected of discordant type of chromosomal aberrations and were considered independent clonal origin. And both of them were metachronous tumors, and the primary tumor and subsequent tumor had an interval of more than 5 years.Conclusion: Multifocal urothelial carcinomas are mostly of monoclonal origin. Some metachronous tumors are most probably polyclonal origin and always with a long intervals.
- urothelial carcinoma /
- fluorescence in situ hybridization /
- clonality /
- multifocality /
- recurrence

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参考文献(14)

[1]	Sung M T, Zhang S, Gregory T, et al. Histogenesis of clear cell adenocarcinoma in the urinary tract:evidence of urothelial origin[J]. Clin Cancer Res, 2008, 14:1947-1955.
[2]	Hǒglund M. On the origin of syn-and metachronous urothelial carcinomas[J]. Eur Urol, 2007, 51:1185-1193.
[3]	Harris A L, Neal D E. Bladder cancer-field versus clonal origin[J]. N Engl J Med, 1992, 326:759-761.
[4]	Hafner C, Knuechel R, Zanardo L, et al. Evidence for oligoclonality and tumor spread by intraluminal seeding in multifocal urothelial carcinomas of the upper and lower urinary tract[J]. Oncogene, 2001, 20:4910-4915.
[5]	Hafner C, Knuechel R, Stoehr R, et al. Clonality of multifocal urothelial carcinomas:10 years of molecular genetic studies[J]. Int J Cancer, 2002, 101:1-6.
[6]	Jones T D, Wang M, Eble J M, et al. Molecular evidence supporting field effect in urothelial carcinogenesis[J]. Clin Cancer Res, 2005, 11:6512-6519.
[7]	Hurst C D, Fiegler H, Carr P, et al. High-resolution analysis of genomic copy number alterations in bladder cancer by microarray-based comparative genomic hybridization[J]. Oncogene, 2004, 23:2250-2263.
[8]	Schlomer B J, Ho R, Sagalowsky A, et al. Prospective validation of the clinical usefulness of reflex fluorescence in situ hybridization assay in patients with atypical cytology for the detection of urothelial carcinoma of the bladder[J]. J Urol, 2010, 183:62-67.
[9]	Veeramachaneni R, Nordberg M L, Shi R, et al. Evaluation of fluorescence in situ hybridization as an ancillary tool to urine cytology in diagnosing urothelial carcinoma[J]. Diagn Cytopathol, 2003, 28:301-307.
[10]	Gallucci M, Guaraní F, Marzano R, et al. Status of the p53, p16, RB1, and HER-2 genes and chromosomes 3, 7, 9 and 17 in advanced bladder cancer:correlation with adjacent mucosa and pathological parameters[J]. J Clin Pathol, 2005, 58:367-371.
[11]	Phillips J L, Richardson I C. Anenploidy in bladder cancer:the utility of fluorescent in situ hybridization in clinical practice[J]. BJU Int, 2006, 98:33-37.[ZK)]
[12]	Jones T D, John N, Eble J N, et al. Molecular genetic evidence for the independent origin of multifocal papillary tumors in patients with papillary renal cell carcinomas[J]. Clin Cancer Res, 2005, 11:7226-7233.
[13]	Ishiwata S, Takahashi Y, Tanka Y, et al. Noninvasive detection and prediction of bladder cancer by fluorescence in situ hybridization analysis exfoliated urothelia cell in voided urine[J]. J Urol, 2001, 57:811-815.
[14]	Boolmann M, Heller H, Bankfalvi A, et al. Quantitative molecular urinary cytology by fluorescence in situ hybridization:a tool for tailoring surveillance of patients with superficial bladder cancer[J]. BJU Int, 2005, 95:1219-1225.